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| 产品名称： | pLIN101 |
|---|---|
| 商品货号： | TS168215 |
| Designations： | pLIN101 |
| GenBank Number： | U15050 |
| Species： | Streptococcus agalactiae Lehmann and Neumann |
| Depositors： | DG Pritchard, B Lin |
| Applications： | produces protein hyaluronate lysase |
| Vector： | Construct size (kb): 7.931000232696533 |
| Insert： | DNA: genomic Insert lengths(kb): 3.420000076293945 Gene product: hyaluronate lysase hylB Target Gene: hyaluronate lysase |
| Insert Size (kb)： | 3.420 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--8.0; XbaI--8.0; EcoRI--8.0; BamHI--8.0; SalI/XbaI--4.6, 3.4. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.35, 0.88; KpnI--1.2; EcoRI--2.19; ClaI--3.22. The insert excised with SalI/XbaI will include some vector sequence: 12 bp at the 5 end (SalI) and 36 bp at the 3 end (XbaI). |
| References： | Lin B, et al. Cloning and expression of the gene for group B streptococcal hyaluronate lyase. J. Biol. Chem. 269: 30113-30116, 1994. PubMed: 7982914 Pritchard DG, et al. Characterization of the group B streptococcal hyaluronate lyase. Arch. Biochem. Biophys. 315: 431-437, 1994. PubMed: 7986088 |