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| 产品名称： | TIME-GFP |
|---|---|
| 商品货号： | TS168270 |
| Organism： | Homo sapiens, human |
| Tissue： | foreskin |
| Cell Type： | microvascular endothelial cell |
| Product Format： | frozen |
| Morphology： | endothelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | neonatal |
| Gender： | male |
| Applications： | Both TIME-GFP (TS168270) and its parental cell line TIME (ATCC CRL-4025) exhibit normal diploid karyotype, extended lifespan in culture and endothelial characteristics that make them an ideal model for investigating many aspects of endothelial cell biology. TIME-GFP (TS168270) retains angiogenic potential and stably expresses green fluorescence protein (GFP) for at least 12 passages. The GFP-expressing cells migrate and coalesce into networks of vessel-like structure within 8 hours after being plated onto basement membrane gel (CellMatrix, ATCC ACS-3035). The stable expression of GFP in these cells enables detection and analysis of the fragile endothelial structures to occur without post-assay fixation and/or staining. |
| Shipping Information： | FZ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a diploid cell line of male origin with a modal chromosome number of 46 and a low rate of polyploidy. The line shows some karyotypic instability at later passages. |
| Images： | 
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| Derivation： | TIME-GFP (TS168270) is derived by transfecting TIME (ATCC CRL-4025) cells with linearized pWE2-EmGFP plasmid, which constitutively expresses the Green Fluorescence Protein (GFP) driven by a CMV promoter. xa0 A stable GFP-expressing clone is selected to establish the TIME-GFP cell line. |
| Clinical Data： | male neonatal |
| Antigen Expression： | Positive for CD31 and capable of uptaking Low Density Lipoprotein (LDL). Express Green Fluorescence Protein (GFP) constitutively |
| Complete Growth Medium： | The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040) OR Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041). Add Blasticidin (Life Technologies Cat. No. A11139-03) at a final concentration of 12.5 ug/mL and G418 (Life Technologies Cat. No. 11811-023) at a final concentration of 200 ug/mL. Note: Do not filter complete medium.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes. Subculture when the culture is about 90% confluent.
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| Cryopreservation： | 90% FBS, 10% DMSO |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | D5S818: xa011 D13S317: 9, 11 D7S820: 8, 9 ; D16S539: 9, 12 vWA: 16, 18 Amelogenin: X, Y TPOX: 8 CSF1PO: 11, 12 TH01: 6, 7 |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor： | ATCC |