微信小陈
微信小章| 产品名称： | pSK-hGCS1 |
|---|---|
| 商品货号： | TS168324 |
| Designations： | pSK-hGCS1 |
| GenBank Number： | M90656 |
| Species： | Homo sapiens, human |
| Depositors： | R Mulcahy |
| Applications： | The 764 bp PstI fragment (derived from the open reading frame) has been used as a probe for the human and rat sequences. |
| Vector： | Construct size (kb): 4.639999866485596 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II SK Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, T7, T3 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GCS* Gene name: gamma-glutamylcysteine synthetase Contains complete coding sequence?: U Tissue: kidney Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.679 Cross references: DNA Seq. Acc.: M90656 Insert lengths(kb): 1.679000020027161 Tissue: kidney Gene product: gamma-glutamylcysteine synthetase( glutamylcysteine synthetase, gamma, gamma-glutamylcysteine synthetase, light subunit) GCS* |
| Insert Size (kb)： | 1.679 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.65; BglII--4.65; HindIII--4.65; PvuII--2.45, 1.45, 0.75; PstI--3.7, 0.8, 0.15. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.463; NcoI--0.65; PstI--0.14, 0.9; PvuII--0.44; SacI--0.9; XbaI--1.44. The first 120 bp of the insert do not match the corresponding sequences of the full-length cDNA and presumably represent cloning artifacts. Detects human transcripts of 4.0 and 3.2 kb. The 764 bp PstI fragment (derived from the open reading frame) has been used as a probe for the human and rat sequences. Encodes 387 aa (of 637 aa) at the carboxyl end of the protein, and includes 398 nt of 3 untranslated sequence. The portion of this clone derived from the cDNA therefore corresponds approximately to nt 845 - 2400 of the sequence record. |
| References： | Gipp JJ, et al. Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase. Biochem. Biophys. Res. Commun. 185: 29-35, 1992. PubMed: 1350904 Bailey HH, et al. Increase in gamma-glutamylcysteine synthetase activity and steady-state messenger RNA levels in melphalan-resistant DU-145 human prostate carcinoma cells expressing elevated glutathione levels. Cancer Res. 52: 5115-5118, 1992. PubMed: 1355406 R. Timothy Mulcahy, personal communication |