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| 产品名称： | pc-fos (mouse)-3 |
|---|---|
| 商品货号： | TS168407 |
| Designations： | pc-fos (mouse)-3 |
| Species： | Mus musculus, mouse |
| Depositors： | T Curran |
| Applications： | The 3 EcoRI/EcoRI fragment (5.4 kb) used in constructing this clone contains 3 of the 4 exons. |
| Vector： | Construct size (kb): 11.19999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 7.099999904632568 Tissue: liver/placenta Gene product: FBJ murine osteosarcoma viral (v-fos) oncogene homolog( FBJ murine osteosarcoma viral (v-fos) oncogene homolog, FOS) Fos |
| Insert Size (kb)： | 7.100 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): Sst--5.1, 3.7, 2.8,0.4; XhoI--11.8; EcoRI--6.6, 5.4; EcoRI/Sst--4.4, 3.0, 2.0, 1.75, 0.56; EcoRI/SalI--6.0, 3.8, 1.8, 0.68. The 3 EcoRI/EcoRI fragment (5.4 kb) used in constructing this clone contains 3 of the 4 exons. Constructed from lambdac-fos(mouse)-2 (5 Sst/EcoRI) and pc-fos (mouse)-1 (3 EcoRI/EcoRI) to make a complete c-fos gene. The insert contains internal HindIII, XhoI, EcoRI, NcoI, SstI, SalI, and BamHI sites. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Curran T, et al. Structure of the FBJ murine osteosarcoma virus genome: Molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells. Mol. Cell. Biol. 3: 914-921, 1983. PubMed: 6306448 Miller AD, et al. c-fos protein can induce cellular transformation: a novel mechanism of activation of a cellular oncogene. Cell 36: 51-60, 1984. PubMed: 6607118 |