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微信小章| 产品名称： | liver 10 cDNA liver 10 |
|---|---|
| 商品货号： | TS168429 |
| Designations： | liver 10 cDNA liver 10 |
| Species： | Mus musculus, mouse |
| Depositors： | JH Weis |
| Applications： | When the 340 bp BamHI/BamHI fragment (lacking the extra 129 nucleotides) is used as a probe, the following EcoRI fragments are detected in BALB/c mice: 7.5 kb, 5.6 kb (chromosome 8), and 4.5 kb. |
| Vector： | Construct size (kb): 6.150000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.781999945640564 Tissue: liver Gene product: complement receptor related protein Crry |
| Insert Size (kb)： | 1.782 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.7; HindIII--5.6, 0.5; BamHI--4.3, 1.2, 0.3; PstI--6.0. The insert contains the following sites (nucleotide position according to the published sequence): BamHI--514, 848; HindIII--1240, XbaI--1480. Crry and Cr-2 are within 10 kb of each other and are transcribed in the same direction. Encodes a single open reading frame starting at nt 482 of the published sequence and extending through the 3 end of the cDNA. Comparison with genomic sequence shows this clone lacks sequences for 7 additional amino acids. Spleen 5 cDNA (ATCC 63065) differs from liver 10 cDNA (TS168429) by having an additional 129 nucleotides between the signal sequence and the nucleotides encoding the first 60 aa repeat. Spleen 5 also does not extend as far in the 5 direction. Liver 10 cDNA and spleen 5 cDNA are clones of alternative splice products of the Crry gene. The mRNAs are transcribed in all tissues examined in a 10 (liver 10) to 1 (spleen 5) ratio. When the 340 bp BamHI/BamHI fragment (lacking the extra 129 nucleotides) is used as a probe, the following EcoRI fragments are detected in BALB/c mice: 7.5 kb, 5.6 kb (chromosome 8), and 4.5 kb. Detects mRNA species of 1.9, 2.0, and 2.1 kb. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Paul MS, et al. The murine complement receptor gene family III: the genomic and transcriptional complexity of the Crry and Crry-ps genes. J. Immunol. 144: 1988-1996, 1990. PubMed: 2307848 Paul MS, et al. The murine complement receptor family. Analysis of mCRY gene products and their homology to human CR1. J. Immunol. 142: 582-589, 1989. PubMed: 2911011 Kingsmore SF, et al. Genetic organization of complement receptor-related genes in the mouse. J. Exp. Med. 169: 1479-1484, 1989. PubMed: 2564419 Utani A, et al. Mouse kalinin B1 (laminin beta 3 chain): cloning and tissue distribution. Lab. Invest. 72: 300-310, 1995. PubMed: 7898049 |