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| 产品名称： | pBC12BI 4053B |
|---|---|
| 商品货号： | TS168435 |
| Designations： | pBC12BI 4053B |
| Depositors： | Hoffmann-La Roche Ltd., PC Watkins, Hoffmann-La Roche Ltd. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0700001716613770 Vector: pBC12BI (plasmid) Promoters: Promoter RSV LTR Construction: pXF3, pBC81delta4, SV40 Marker(s):ampR Construct size (kb): 4.099999904632568 Features: marker(s): ampR promoter: RSV LTR replicon: pMB1, SV40 terminator: rat insulin II enhancer: RSV LTR |
| Applications： | contains sequence preproinsulin 2 expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRI--2.8, 0.78, 0.44; HindIII--4.1; BstEII--4.1. This was constructed from pXF3 (pBR322 minus the poison sequence), a SV40 ori, and a rat preproinsulin genomic sequence under the control of RSV LTR (pBC81delta4 cut at BgIII and BalI and inserted at the BamHI and ClaI sites). cDNA sequences with a translation initiation codon are usually inserted between the unique HindIII and BamHI sites (5 to 3). Polyadenylation signals (and possibly carboxy terminal amino acids) are contributed by the preproinsulin gene. Genomic sequences are normally inserted between unique HindIII and SmaI sites (5 to 3). This deletes the preproinsulin gene from the vector. cDNA clones without an initiation codon may be cloned in frame into the unique BamHI site 3 to the AUG of the rat preproinsulin gene. This results in 5 - 6 additional amino terminal amino acids from the insulin signal peptide. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cullen BR. Use of eukaryotic expression technology in the functional analysis of cloned genes. Methods Enzymol. 152: 684-704, 1987. PubMed: 3657593 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |