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| 产品名称： | Encephalomyocarditis virus |
|---|---|
| 商品货号： | TS168453 |
| Classification： | Picornaviridae, Cardiovirus |
| Agent： | Encephalomyocarditis virus |
| Strain： | MM |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This item was deposited at ATCC® after six passages in weanling mice and three passages in suckling mice. A seed stock was produced by a single passage in BHK-21 cells. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | BHK-21 (ATCC® CCL-10™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-3 days |
| Name of Depositor： | SE Grossberg, Medical College of Wisconsin |
| Source： | Isolated from dead mouse found in the basement of a patient with poliomyelitis, New York, 1942 |
| References： | Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945. Oberste MS, et al. Human febrile illness caused by encephalomyocarditis virus infection, Peru. Emerg. Infect. Dis. 15: 640-646, 2009. PubMed: 19331761 Pindak FF, Schmidt JP. Propagation of MM virus in continuous cell lines. Appl. Microbiol. 17: 815-818, 1969. PubMed: 4307881 |