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微信小章| 产品名称： | pTB10.163 |
|---|---|
| 商品货号： | TS168508 |
| Designations： | pTB10.163 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | RFLPs were observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.700000047683716 Gene product: DNA Segment, single copy D10S22 Alleles: A3, A1, A2 |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.6; HindIII--5.6; EcoRI/HindIII--2.8, 2.8. Codominant segregation demonstrated in 47 3-generation families for the MspI RFLP. RFLPs were observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: TaqI, RsaI, PstI, BglII, PvuII. |
| References： | Wu JS, et al. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am. J. Hum. Genet. 46: 624-630, 1990. PubMed: 1968709 White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 Bragg T, et al. Isolation and mapping of a polymorphic DNA sequence (pTB10.163) on chromosome 10 D10S22. Nucleic Acids Res. 16: 4185, 1988. PubMed: 2453849 |