Microvascular Endothelial Cell Growth Kit-VEGF

货号	TS168534
中文名称	null
适应种属
检测范围
微信小陈	微信小章
在线留言

产品简介

购买须知

产品名称：

Microvascular Endothelial Cell Growth Kit-VEGF

商品货号：

TS168534

Biosafety Level：

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format：

frozen 1 kit

Storage Conditions：

-20°C (or -70°C for long-term storage)

Comments：

The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:

rh VEGF: 5 ng/ml
rh EGF: 5 ng/ml
rh FGF basic: 5 ng/ml
rh IGF-1: 15 ng/ml
L-glutamine: 10 mM
Heparin sulfate: 0.75 Units/ml
Hydrocortisone: 1 µg/ml
Ascorbic acid: 50 µg/ml
Fetal bovine serum: 5%xa0

Complete Growth Medium：

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC^® PCS-110-040), add the indicated volume for each component:xa0

Component

Volume：

Volume

Sterility Tests：

Final Concentration

Functional Tests：

Bovine Brain Extract (BBE)

C of A：

1.0 mL

序号	文章标题	操作
1	ATCC细胞运输和处理	查看详情
2	其他海外品牌代理	查看详情
3	ATCC代理	查看详情
4	购买须知	查看详情
5	订购流程	查看详情

宁波泰斯拓生物

产品中心

Microvascular Endothelial Cell Growth Kit-VEGF

研发产品

技术服务

新闻中心

技术资源

关于我们