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| 产品名称： | pMMB67HE |
|---|---|
| 商品货号： | TS168538 |
| Designations： | pMMB67HE |
| Depositors： | E Lanka |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.84 Vector: pMMB67HE (plasmid) Promoters: Promoter tac Construction: pMMB66HE (ATCC 376210), M13mp19 polylinker Marker(s):ampR Construct size (kb): 8.84 Features: marker(s): ampR promoter: tac replicon: RSF1010 (IncQ) repressor gene: lacIq transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Contains the following sites separated by (bp): EcoRI-739 -AhaIII-97 -ScaI-112 -PvuI-2101 -AccI-1848 -ScaI-55 -AccI-2039 -BstEII-372 -PvuII-93 -PvuII-94 -HpaI-320 -BstEII-182 -MluI-788 -EcoRI. Restriction digests of the clone give the following sizes (kb): AccI--3.9, 3.0, 1.9; BamHI--8.8; HindIII/PvuII--7.6, 1.1. In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623) vector series, the EH and HE in the name designate the orientation of the multiple cloning site to the tac promoter. An autoregulated high-level expression vector utilizing the tac promoter, rrnB terminators and lacIq for lac repression. Concentration of induced gene product may be regulated by varying the lac inducer concentration. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. The M13mp9 polylinker of pMMB66HE (ATCC 37621) was replaced with the M13mp19 polylinker. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Jon A Kornacki, personal communication Erich Lanka, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |