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| 产品名称： | p345 |
|---|---|
| 商品货号： | TS168585 |
| Designations： | p345 |
| GenBank Number： | J00129 |
| Species： | Homo sapiens, human |
| Depositors： | ST Lord |
| Applications： | The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.399999976158142 Tissue: liver Gene product: fibrinogen, B beta polypeptide FGB Alleles: C1, C2 |
| Insert Size (kb)： | 1.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI--6.8; EcoRI/PstI--4.9, 1.9; HpaI--6.8; BglII--6.8. The insert contains HpaI and BglII sites. The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region. The ability of this probe to detect the BclI polymorphism was verified by Jeffrey Murray, University of Iowa. Originally cloned as a PstI fragment. The beta-coding sequence was subcloned and the 5 end manipulated with linkers to add a cistron. The encoded protein begins at the first amino acid of the circulating protein. |
| References： | Chung DW, et al. Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta-chain of human fibronogen. Biochemistry 22: 3244-3250, 1983. PubMed: 6688356 Bolyard MG, Lord ST. Expression of Escherichia coli of the human fibrinogen B beta chain and its cleavage by thrombin. Blood 73: 1202-1206, 1989. PubMed: 2649171 Arthur L Beaudet, personal communication Susan T Lord, personal communication |