微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUC18CMR |
|---|---|
| 商品货号： | TS168693 |
| Designations： | pUC18CMR |
| Depositors： | HP Schweizer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.2729997634887700 Vector: pUC18CMR (plasmid) Promoters: Promoter none Construction: pUC18, pUC4K, pBR325 Marker(s):ampR,cmlR Construct size (kb): 4.27299976348877 Features: marker(s): ampR, cmlR promoter: none replicon: pMB1 terminator: none enhancer: none |
| Applications： | contains easily purifiable cassette(s) for construction |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 0.89; PstI--4.27; EcoRI/HindIII--2.45, 0.89, 0.75. The cml cassette can be isolated by digestion with one enzyme (BamHI), by double digestion yielding blunt ends (HincII/SmaI), or by double digestion yielding overhangs for directional cloning. pUC18CML (ATCC 37717) and pUC18CMR (TS168693) differ only in the orientation of the cml cassette. In pUC18CMR, the lac promoter and the cml gene are in the same orientation. A plasmid containing the gene determining chloramphenicol resistance which is flanked by polylinker sites for ease in manipulation. cmlR sequences (pBR325 cut with BclI/blunt ended with Klenow/cut with HincII) were ligated to a 2.6 kb HincII fragment of pUC4K. The 1.6 kb BamHI fragment was cloned into M13mp18; a 1.6 kb KpnI/HindIII fragment was cloned into the BamHI site of pUC18. |
| Media： | ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schweizer HP. The pUC18CM plasmids: a chloramphenicol resistance gene cassette for site-directed insertion and deletion mutagenesis in Escherichia coli. BioTechniques 8: 612-616, 1990. PubMed: 2192739 |
| Shipped： | freeze-dried |