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| 产品名称： | pCN50 |
|---|---|
| 商品货号： | TS168772 |
| Designations： | pCN50 |
| Depositors： | R Diaz Orejas |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.5000000000000000 Vector: pCN50 (plasmid) Construction: pCN38, pBR322 Marker(s):ampR,kanR,tetR Construct size (kb): 8.5 Features: marker(s): kanR, ampR, tetR replicon: repA, pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 3.1; HindIII/PstI--4.5, 3.4, 0.8; PvuII--4.4 (doublet); PvuII/KpnI--4.4, 2.5, 1.6. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences. Multiple selectable markers permit insertional activation to screen for recombinants (PstI site in ampR). Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans. Constructed by ligating pCN38 (ATCC 77095) and pBR322 which had been linearized with PvuII. The order of the major features in this plasmid is: PvuII - pBR322 (pMB1 ori - bla - tetR) - PvuII - BamHI - kanR - HindIII - repA. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095 Ramon Diaz Orejas, personal communication |
| Shipped： | frozen |