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| 产品名称： | lambdaSE6 |
|---|---|
| 商品货号： | TS168775 |
| Designations： | lambdaSE6 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 46.0 Vector: lambdaSE6 (phagemid) Construction: lambdaSE4, pSE106 Marker(s):ampR,kanR Construct size (kb): 46.0 Features: insert detection: Spi+ marker(s): ampR, kanR replicon: pMB1, NR1, lambda repressor gene: cI857 |
| Applications： | vector permitting positive selection for inserts |
| Comments： | Plasmid is temperature inducible to produce infectious phage particles. Designed to identify genes by complementation of E. coli host mutations in hosts producing lambda cI protein. Accepts inserts of 6-24 kb. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Elledge SJ, Walker GC. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants. J. Bacteriol. 162: 777-783, 1985. PubMed: 2985547 |
| Shipped： | freeze-dried |