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| 产品名称： | pG59/T1 C151/T1, C151/L1-T1 |
|---|---|
| 商品货号： | TS168815 |
| Designations： | pG59/T1 C151/T1, C151/L1-T1 |
| GenBank Number： | J03496 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 21.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 11.0 Gene product: leucyl tRNA synthetase, mitochondrial MSL1 |
| Insert Size (kb)： | 11.0 |
| Genotype： | MATalpha leu2-3 leu2-112 msl1-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (approximate kb): BamHI- 0.24- PstI- 0.45- BamHI- 1.0- BamHI- 0.63- XhoI- 0.29- PstI- 2.3- XbaI- 1.0- BamHI- 0.6- XbaI- 1.1- XbaI- 2.9- BamHI- 0.25- BamHI. Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--11, 3.7, 3.3, 2.2, 1.05, 0.94; KpnI--uncut; XhoI--15, 7; XbaI--16, 4.2, 1.45; PvuII--17, 5.1. The insert includes a continuous open reading frame of 2682 nt encoding a protein of 894 amino acids and a molecular weight of 102 kDa. The probe, pG59/T1, has been deposited in Escherichia coli RR1 as TS168815 and in Saccharomyces cerevisiae C151/L1 as ATCC 66312. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Tzagoloff A, et al. Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli. J. Biol. Chem. 263: 850-856, 1988. PubMed: 2826465 |