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| 产品名称： | WT 9-12 |
|---|---|
| 商品货号： | TS168844 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney; cyst from a distal and proximal cortical tubule |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | polygonal |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | autosomal dominant polycystic kidney disease (ADPKD) |
| Age： | unknown |
| Gender： | female |
| Applications： | WT 9-12 is one of a series of immortalized epithelial cell lines from over 30 individual renal cysts obtained from 11 patients with ADPKD. These new series of cyst-derived cell lines represent useful in vitro models for studying the cellular and molecular biology of ADPKD.
Antigen: SV40 large T antigen, expressed PubMed: 12734101 The WT 9-12 cell line was established in July, 1999 from a single cyst from a cortical tubule taken from a patient with autosomal dominant polycystic kidney disease (ADPKD). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The WT 9-12 cell line was established in July, 1999 from a single cyst from a cortical tubule taken from a patient with autosomal dominant polycystic kidney disease (ADPKD). The cells have both distal and proximal characteristics. They can be subcloned to obtain clones with proximal or distal markers. The cells were transformed with a wild type recombinant adeno-SV40 virus. These viruses contain an origin-deficient (ori-) WT SV40 DNA cloned into the adenovirus vector in place of regions 1a and 1b PubMed: 12734101.
WT 9-12 is one of a series of immortalized epithelial cell lines from over 30 individual renal cysts obtained from 11 patients with ADPKD. These new series of cyst-derived cell lines represent useful in vitro models for studying the cellular and molecular biology of ADPKD.
Antigen: SV40 large T antigen, expressed PubMed: 12734101 |
| Clinical Data： | The WT 9-12 cell line was established in July, 1999 from a single cyst from a cortical tubule taken from a patient with autosomal dominant polycystic kidney disease (ADPKD). female |
| Antigen Expression： | alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens, expressed Ref   Loghman-Adham M, et al. Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts. Am. J. Physiol. Renal Physiol. 285: F397-F412, 2003. PubMed: 12734101 |
| Receptor Expression： | epidermal growth factor (EGF), expressed Ref Loghman-Adham M, et al. Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts. Am. J. Physiol. Renal Physiol. 285: F397-F412, 2003. PubMed: 12734101 |
| Genes Expressed： | epithelial sodium channel, ENaC, alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens, expressed |
| Cellular Products： | epithelial sodium channel, ENaC |
| Comments： | The WT 9-12 cell line was established in July, 1999 from a single cyst from a cortical tubule taken from a patient with autosomal dominant polycystic kidney disease (ADPKD). The cells have both distal and proximal characteristics. They can be subcloned to obtain clones with proximal or distal markers. The cells were transformed with a wild type recombinant adeno-SV40 virus. These viruses contain an origin-deficient (ori-) WT SV40 DNA cloned into the adenovirus vector in place of regions 1a and 1b PubMed: 12734101.
WT 9-12 is one of a series of immortalized epithelial cell lines from over 30 individual renal cysts obtained from 11 patients with ADPKD. These new series of cyst-derived cell lines represent useful in vitro models for studying the cellular and molecular biology of ADPKD.
Antigen: SV40 large T antigen, expressed PubMed: 12734101 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Flasks must be coated with Bovine Collagen type I solution (supplied stock concentration is 3.0 mg/mL; PureCol® Advanced Biomatrix Catalog No. 5005-B) for 1 hour at 37C.
Interval: Maintain cultures at a cell concentration between 5 X 10(3) and 8 X 10(4) cells/sq. cm
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time： | 38 hours |
| Name of Depositor： | M Loghman-Adham |
| Year of Origin： | July, 1999 |
| References： | Loghman-Adham M, et al. Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts. Am. J. Physiol. Renal Physiol. 285: F397-F412, 2003. PubMed: 12734101 |