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| 产品名称： | lambdaF1 |
|---|---|
| 商品货号： | TS168856 |
| Designations： | lambdaF1 |
| GenBank Number： | M12949 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc, TH Rabbitts |
| Vector： | Construct size (kb): 45.0 |
| Insert： | DNA: genomic Insert lengths(kb): 15.5 Tissue: F8 cell line (T-prolymphocytic leukemia) Gene product: T cell receptor, gamma, joining segment JP TCRGJ1 |
| Insert Size (kb)： | 15.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI-->23.0, 9.2, 1.7; XbaI--20.0, 13.5, 9.2, 4.6; SacI--20.0, 9.2, 5.0 (doublet), 3.0, 1.9, 1.7; XhoI--20.0, 16.0, 9.2, 2.0; PvuI-->23.0, 12.0, 8.2. The insert contains the following restriction sites (approximate kb from the 5 end): SacI--2.9, 4.5, 9.1, 13.6, 15.5 (polylinker). Contains the pseudogene Vgamma1 and a non-productive rearrangement of Vgamma2 and JgammaP. The insert begins approximately 5.0 kb upstream of pseudogene Vgamma1 and extends 1.6 kb beyond Jgamma1. Additional GenBank/EMBL accession numbers: M12951, M13429, M12960, X03434. |
| References： | LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743 Marie-Paule Lefranc, personal communication |