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| 产品名称： | pPYRDH |
|---|---|
| 商品货号： | TS168864 |
| Designations： | pPYRDH |
| GenBank Number： | M94065 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein dihydroorotate dehydrogenase |
| Vector： | Construct size (kb): 6.929999828338623 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pFL61 Intact vector size: 5.425 Type of vector: plasmid Vector end: BstXI Vector end: BstXI Cloning sites: NotI Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI Construction: pUC19 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3 replicon: pMB1, 2 micron Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DHODH Gene name: dihydroorotate dehydrogenase Contains complete coding sequence?: Y Localization: 16 q22 Tissue: HeLa D98-H2 cell line Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.5 Cross references: DNA Seq. Acc.: M94065 Insert lengths(kb): 1.5 Tissue: HeLa D98-H2 cell line Gene product: dihydroorotate dehydrogenase DHODH Target Gene: dihydroorotate dehydrogenase |
| Insert Size (kb)： | 1.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--5.5, 1.5; BamHI--4.6, 2.3; BglI--3.6; 1.9, 1.1, 0.4; PvuII--4.8, 2.1; ClaI--4.2, 2.35, 0.5. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.42; BglI--0.87, 1.25; ClaI--0.83; PstI--0.14, 0.29, 0.71; PvuII--1.06; SphI--1.3. Contains the complete coding sequence (nt 1-1191). Can complement ura1 mutants in Saccharomyces cerevisiae. Detects a unique 1.6 kb transcript in humans. |
| References： | Minet M, et al. Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant. Gene 121: 393-396, 1992. PubMed: 1446837 |