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| 产品名称： | pSHT |
|---|---|
| 商品货号： | TS168925 |
| Designations： | pSHT |
| Depositors： | P Bird |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0000000000000000 Vector: pSHT (phagemid) Promoters: Promoter T7 Construction: pSVT7 Marker(s):ampR Construct size (kb): 4.0 Features: marker(s): ampR promoter: SV40 early, T7 replicon: pMB1, M13, SV40 |
| Applications： | expression vector vector permitting production of single-stranded DNA vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--4.0; BamHI--4.0; HindIII--4.0. Shuttle expression vector providing a start codon and signal sequence (from influenza virus hemagglutinin HA) necessary to express membrane and secretory proteins in mammalian cells. Contains stop codons in all three frames after cloning sites. The order of the major features in the phagemid is: pMB1 ori - ampR - M13 ori - ClaI - SV40 ori - SV40 early promoter - T7 promoter - HindIII - HA signal sequence - BamHI/SmaI/SpeI - termination signals - EcoRI - NheI - SV40 intron - BclI - SV40 polyadenylation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Madison EL, Bird P. A vector, pSHT, for the expression and secretion of protein domains in mammalian cells. Gene 121: 179-180, 1992. PubMed: 1427094 Bird P, et al. Translocation in yeast and mammalian cells: not all signal sequences are functionally equivalent. J. Cell Biol. 105: 2905-2914, 1987. PubMed: 3320059 |
| Shipped： | freeze-dried |