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| 产品名称： | SQMK-FP |
|---|---|
| 商品货号： | TS168994 |
| Organism： | Saimiri boliviensis boliviensis, monkey, bolivian squirrel |
| Tissue： | kidney; tubule |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 1 day newborn |
| Gender： | male |
| Applications： | The cell line can be used to study the biology of the squirrel monkey in vitro. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey. SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | hyperdiploid with chromosome numbers ranging from 52 to 57 (submetacentric chromosome pair 15 and acrocentric chromosome pair 16).
Ref  Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921 |
| Images： |  |
| Derivation： | SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey.
The cell line can be used to study the biology of the squirrel monkey in vitro. |
| Clinical Data： | male |
| Comments： | SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey.
The cell line can be used to study the biology of the squirrel monkey in vitro. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Protocol:
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 2 X 10(6) cells/cm2. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every two to three days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time： | approximately 22 hours |
| Name of Depositor： | JG Scammell |
| Deposited As： | Saimiri boliviensis boliviensis |
| Year of Origin： | 1997 |
| References： | Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921 Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921 |