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| 产品名称： | pPFZ-R2 |
|---|---|
| 商品货号： | TS169045 |
| Designations： | pPFZ-R2 |
| Species： | Bos taurus, cow |
| Depositors： | Pfizer, Inc., LH Huang, Pfizer, Inc. |
| Applications： | produces protein prorennin A prochymosin |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: plasmid Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: bovine Gene name: prorennin A Contains complete coding sequence?: U Type of DNA: cDNA Insert end: Insert end: Insert size (kb): Cross references: Gene product: prorennin A(prochymosin) Target Gene: prorennin A |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--4.4, 0.5, 0.4; KpnI/SalI--4.5, 1.0. pPFZ-R2 (TS169045) and pPFZ-R4 (ATCC 39543) are the same except that the ATG initiation codon follows the ribosomal binding site of the trp leader peptide by 5 bp in pPFZ-R2 and by 11 bp in pPFZ-R4. The insert contains BamHI sites. A KpnI/SalI digest should give a probe of about 970 bp which contains the sequences encoding aa 83 through the TGA stop codon. pPFZ-R2 (TS169045) shows significantly higher expression of the protein product than pPFZ-R4 (ATCC 39543). Constructed from two fragments of pCR101, a 235 bp BamHI/KpnI fragment (encoding aa 6-83) and a 4772 bp HindIII/KpnI fragment (encoding aa 83 through TGA stop codon, pMB1, ampR) ligated with a 370 bp HindIII/BamHI fragment of ptrpLI-R2-B48. The 370 bp HindIII/BamHI fragment of ptrpLI-R2-B48 contains trp promoter-operator, ribosome binding site, artificial ATG initiation codon, and chemically derived sequence encoding the first 5 amino acids of the protein. |
| References： | Franke AE. Expression plasmids for improved production of heterologous protein in bacteria. US Patent 4,935,370 dated Jun 19 1990 Nishimori K, et al. Expression of cloned calf prochymosin gene sequence in Escherichia coli. Gene 19: 337-344, 1982. PubMed: 6295885 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |