微信小陈
微信小章
产品简介
购买须知
| 产品名称： | cCJ52.1 |
|---|---|
| 商品货号： | TS169056 |
| Designations： | cCJ52.1 |
| Species： | Homo sapiens, human |
| Depositors： | C Julier |
| Vector： | Construct size (kb): 48.0 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D16S152 Alleles: A1, B1, A2, B2 |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--10.0, 9.4, 5.6, 4.2, 3.5, 3.0, 1.9, 1.7, 1.5, 1.3, 1.2, 0.8, 0.5; SalI-->23.1, 2.4; AvaI--11.0, 8.8, 5.6, 4.4, 3.3, 2.1 (doublet), 1.9, 1.5, 0.8, 0.7, 0.3; NotI-->23.1, 8.0; BamHI--9.4, 8.4, 6.0, 4.3, 3.8, 3.5, 2.6, 1.9, 1.8, 1.4 (doublet); EcoRI--8.2, 7.8, 6.5, 5.4, 5.2, 4.7, 2.6, 2.2, 1.6, 0.4. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: MspI, PstI. |
| References： | Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986 Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.1) on chromosome 16 D16S152. Nucleic Acids Res. 17: 8014, 1989. PubMed: 2571984 |