宁波泰斯拓生物

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phgJE3-3

货号 TS169065
中文名称 null
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产品名称: phgJE3-3
商品货号: TS169065
Designations: phgJE3-3
GenBank Number:

M26035

Species: Homo sapiens, human
Vector:
Construct size (kb): 3.799999952316284
Insert:
DNA: genomic
Insert lengths(kb): 0.800000011920929
Tissue: embryonic lung fibroblast WI-38 (serum-induced)
Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) JE*
Insert Size (kb): 0.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
The insert contains the following sites (with nucleotide positions according to the published sequence): NaeI at -426; HindIII at -420; SacI at -408 and PstI at -307.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; HindIII--3.65, 0.15; BamHI--3.8; PstI--3.8; XbaI/SacI--2.9, 0.7, 0.2.
The 5 clone phgJE3-3 (ATCC 61366/61367) and the 3 clone phgJE2-1 (ATCC 61368/61369) together generate a complete gene.
This insert was subcloned from lambdahJE-7 and extends from -531 relative to the mRNA cap site into the first intron which begins as +228. It is oriented in this clone with transcription from the SP6 promoter giving the + strand.
References:

Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477

Barrett J Rollins, personal communication