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| 产品名称： | hp35 |
|---|---|
| 商品货号： | TS169196 |
| Designations： | hp35 |
| GenBank Number： | M38443 M65271 |
| Species： | Homo sapiens, human |
| Depositors： | U Gubler |
| Applications： | The cDNA contains two in-frame Met codons: one at position 100-102 and the second one at position 202-204. Only the second Met codon was used for expression of bioactive IL-12 in mammalian cells by the depositor. |
| Vector： | Construct size (kb): 4.064000129699707 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK+ Intact vector size: 2.964 Type of vector: phagemid Vector end: EcoRV Vector end: EcoRV Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IL12A Other gene symbols: IL-12 p35 Gene name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35 Contains complete coding sequence?: Y Chromosome: 3; Localization: 3 p25-p26.2 Type of DNA: cDNA Insert 5 end: EcoRV Insert 3 end: EcoRV Insert size (kb): 1.1 Cross references: DNA Seq. Acc.: M38443 Nucleotides 33 - 1058 of the insert correspond to nucleotides 1 - 1026 of M38443. DNA Seq. Acc.: M65271 Nucleotides 33 - 1058 of the insert correspond to nucleotides 1 - 1026 of M65271. Insert lengths(kb): 1.100000023841858 Gene product: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35 IL12A |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI/SalI--2.9, 1.2; HindIII--3.4, 0.65; EcoRV--4.1. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.42; HindIII--0.63; BglII--0.66. The cDNA contains two in-frame Met codons: one at position 100-102 and the second one at position 202-204. Only the second Met codon was used for expression of bioactive IL-12 in mammalian cells by the depositor. The insert, p35 cDNA including 5 and 3 noncoding regions,was PCR amplified out of a lambda phage clone and ligated into the EcoRV site of pBluescript SK+. |
| References： | Gubler U, et al. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. USA 88: 4143-4147, 1991. PubMed: 1674604 |