微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Bovine adenovirus 1 |
|---|---|
| 商品货号： | TS169277 |
| Classification： | Adenoviridae, Mastadenovirus, Bovine mastadenovirus A |
| Deposited As： | Bovine adenovirus type 1 |
| Agent： | Bovine adenovirus 1 |
| Strain： | 10 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Feces of normal calf |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | REFERENCE STRAIN: Pereira HG et al. A short description of the Adenovirus group. Virology 20: 613-620, 1963. Agglutinates rat RBC. CPE may be minimal, frequently demonstrating less than 25% involvement of cell sheet, even with prolonged incubation.xa0 AAV reported to be present. |
| Effect on Host： | CPE, cell rounding with vacuolization, clumping, and sloughing |
| Recommended Host： |
MDBK (NBL-1) cells (ATCC® CCL-22™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | M Klein |
| Source： | Feces of normal calf |
| References： | Klein M, et al. Isolation from cattle of a virus related to human adenovirus. Proc. Soc. Exp. Biol. Med. 102: 1-4, 1959. PubMed: 14409689 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 |