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| 产品名称： | pEW403 LEW403 |
|---|---|
| 商品货号： | TS169321 |
| Designations： | pEW403 LEW403 |
| Depositors： | DF Barker |
| Vector： | Construct size (kb): 7.30
Name of vector: pUC8Intact vector size: 2.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Construction: pBR322, Escherichia coli lacZalpha, M13mp8 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none |
| Insert： | Genome: Homo sapiens Gene symbol: D17S63 Genomic copy number: unique Gene name: DNA Segment, single copy Contains complete coding sequence?: U Chromosome: 17 Localization: 17 p12-p11.2 Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 4.6 Cross references: Restriction enzyme: MspI Alleles: A2, A1 |
| Insert Size (kb)： | 4.60 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Plasmid in Escherichia coli JM83 |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 2.7; HindIII--5.2, 2.2; BamHI--3.0, 2.5, 2.2; SmaI--7.7; SalI--7.0. This probe contains repetitive sequences. This probe may hybridize to a non 17-specific human fragment, but the polymorphism is linked to 17-specific markers. |
| References： | Wright EC, et al. A genetic map of human chromosome 17p. Genomics 7: 103-109, 1990. PubMed: 2335351 David F Barker, personal communication |