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| 产品名称： | Cafeteria roenbergensis Fenchel and Patterson |
|---|---|
| 商品货号： | TS169326 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | contaminant in CCAP 950/1 culture of Rhizochromulina marina, 1987 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Ultrastructure of flagellar apparatus |
| Medium： | ATCC® Medium 1525: Seawater 802 medium |
| Growth Conditions： | Temperature: 25.0°C Protocol: ATCCNO: 50561 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 10 ml of bacterized ATCC medium 1525 in a T-25 tissue culture flask. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly and incubate horizontally at 25C. The culture should be ready to subculture in 5-7 days. To subculture, vigorously agitate the flask and aseptically transfer a 0.1 ml aliquot to 10 ml of bacterized ATCC medium 1525 containing a single rice grain and incubate as above. ATCC medium 1525 is bacterized by inoculating the medium with a loopful of Klebsiella pneumoniae subsp. pneumoniae ATCC 27889 (a non-pathogenic strain) from an ATCC medium 3 slant approximately 24 hours before using. Other strains of Klebsiella or other species of bacteria may serve equally well as a food source. This must be determined empirically. |
| Subcultivation： | Protocol: ATCCNO: 50561 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 10 ml of bacterized ATCC medium 1525 in a T-25 tissue culture flask. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly and incubate horizontally at 25C. The culture should be ready to subculture in 5-7 days. To subculture, vigorously agitate the flask and aseptically transfer a 0.1 ml aliquot to 10 ml of bacterized ATCC medium 1525 containing a single rice grain and incubate as above. ATCC medium 1525 is bacterized by inoculating the medium with a loopful of Klebsiella pneumoniae subsp. pneumoniae ATCC 27889 (a non-pathogenic strain) from an ATCC medium 3 slant approximately 24 hours before using. Other strains of Klebsiella or other species of bacteria may serve equally well as a food source. This must be determined empirically. |
| Name of Depositor： | CJ OKelly |
| Year of Origin： | 1987 |
| References： | OKelly CJ, Patterson DJ. The flagellar apparatus of Cafeteria roenbergensis Fenchel & Patterson, 1988 (Bicosoecales = Bicosoecida). Eur. J. Protistol. 32: 216-226, 1996. |