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微信小章| 产品名称： | pGEM4Z/SEAP |
|---|---|
| 商品货号： | TS169347 |
| Designations： | pGEM4Z/SEAP |
| Species： | Homo sapiens, human |
| Applications： | If the plasmid is linearized with HindIII, and transcribed from the SP6 promoter in the presence of m7-GpppG, mRNA for the secreted form of placental alkaline phosphatase is produced. mRNA prepared from this construct can be used as an internal standard for expression of mRNA injected into Xenopus oocytes. |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Gene product: alkaline phosphatase, placental (Regan isozyme) ALPP Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII--5.2; BamHI--3.2, 1.8; PstI--3.2, 1.8; SalI--5.2; EcoRI/KpnI--2.9, 2.1. If the plasmid is linearized with HindIII, and transcribed from the SP6 promoter in the presence of m7-GpppG, mRNA for the secreted form of placental alkaline phosphatase is produced. mRNA prepared from this construct can be used as an internal standard for expression of mRNA injected into Xenopus oocytes. Linkage has been established between Waardenburg syndrome type 1 and ALPP in five families (LOD score 4.76 at a recombination fraction of 0.023. Waldenburg syndrome shows close linkage to FN1 and ALPP in one multigenerational family with 11 affected individuals. The described RsaI polymorphism was established using a 1.1 kb EcoRI/ApaI fragment of the ALPP cDNA (ALP-1). Constructed from pBC12/PLAP containing the complete cDNA for the membrane-anchored form by inserting a termination codon after the codon for amino acid 489. The EcoRI/KpnI fragment was then cloned into the vector. |
| References： | Berger J, et al. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene epxression in eukaryotic cells. Gene 66: 1-10, 1988. PubMed: 3417148 Tate SS, et al. Secreted alkaline phosphatase: an internal standard for expression of injected mRNAs in the Xenopus oocyte. FASEB J. 4: 227-231, 1990. PubMed: 2298343 Penhallow RC, Sussman HH. Further characterization of SacI RFLPs at the acid alpha glucosidase (GAA) locus. Nucleic Acids Res. 18: 1930, 1990. PubMed: 1970872 Foy C, et al. Assignment of the locus for Waardenburg syndrome type 1 to human chromosome 2q37 and possible homology to the splotch mouse. Am. J. Hum. Genet. 46: 1017-1023, 1990. PubMed: 2339698 |