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| 产品名称： | pGV1106 |
|---|---|
| 商品货号： | TS169374 |
| Designations： | pGV1106 |
| Depositors： | J Schell, M Van Montagu |
| Other IDs： | Nucleotide (GenBank) : X68227 E.coli plasmid pSa aadA2 gene for streptomycin resistance. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.5000000000000000 Vector: pGV1106 (plasmid) Construction: W-plasmid Sa Marker(s):kanR,spcR,strR Construct size (kb): 10.5 Features: marker(s): kanR, spcR, strR replicon: Sa |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--10.5; EcoRI--10.5; PstI--10.5; BglII--10.5; BamHI/EcoRI--8.0, 2.5; BamHI/BglII--6.2, 4.3. Nonconjugative. Caution: this plasmid is readily lost from the Escherichia coli host. Always grow under antibiotic selection. A broad host range plasmid vector. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Leemans J, et al. Broad-host-range cloning vectors derived from the W-plasmid Sa. Gene 19: 361-364, 1982. PubMed: 6295888 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |