| 产品名称: | pHB20 PHB20 |
|---|---|
| 商品货号: | TS169416 |
| Designations: | pHB20 PHB20 |
| Species: | Homo sapiens, human |
| Depositors: | S Ohta |
| Vector: | Construct size (kb): 4.699999809265137 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.799999952316284 Tissue: HeLa cell line Gene product: ATP synthase, H+ transporting (ATPase, mitochondrial)(ATPase, mitochondrial) ATPM |
| Insert Size (kb): | 1.800 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.62, 1.8; HindIII--4.1; PstI--3.9, 0.47; SalI--4.2; BamHI--3.75, 0.51. Insert contains AatII (67), ScaI (225), TaqI (422), SmaI (692), XbaI (1053), HincII (1206), and KpnI (1296) sites (with numbering system beginning with first amino acid in mature form of subunit). Comparison with genomic sequence demonstrates that this is not a full-length cDNA clone and has rearrangements in the 5 region. |
| References: | Ohta S, Kagawa Y. Human F1-ATPase: Molecular cloning of cDNA for the beta subunit. J. Biochem. 99: 135-141, 1986. PubMed: 2870059 |