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| 产品名称： | pSREBP-1a |
|---|---|
| 商品货号： | TS169486 |
| Designations： | pSREBP-1a |
| GenBank Number： | U00968 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Vector： | Construct size (kb): 7.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II SK+ Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: SalI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SREBF1 Other gene symbols: SREBP1A Gene name: sterol regulatory element binding transcription factor 1 Contains complete coding sequence?: Y Chromosome: 17 Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 4.2 Cross references: DNA Seq. Acc.: U00968 Insert lengths(kb): 4.199999809265137 Gene product: sterol regulatory element binding transcription factor 1( SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1) SREBF1 |
| Insert Size (kb)： | 4.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/SalI--4.2, 3.2; EcoRI--4.3, 2.9; KpnI--6.0, 1.1, 0.4; EcoRI/SalI--2.8, 2.7, 1.5; SacI--6.0, 0.7, 0.5. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.7; KpnI--5.6, 1.2, 0.34; BamHI/SalI--4.2, 2.9; EcoRI/SalI--2.9, 2.7, 1.5; EcoRI--Eco47III--4.4, 1.5, 1.2. The insert contains the following restriction sites (kb from the 5 end): Eco47III--1.174; EcoRI--2.668; KpnI--2.685, 3.021. Constructed with an EcoRI linker at the 5 end and an EcoRI(NotI) adaptor at the 3 end. |
| References： | Yokoyama C, et al. SREBP-1, a basic helix-loop-helix leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell 75: 187-197, 1993. PubMed: 8402897 Joseph L Goldstein, personal communication |