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微信小章| 产品名称： | pFL64+ |
|---|---|
| 商品货号： | TS169489 |
| Designations： | pFL64+ |
| Depositors： | F Lacroute |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.7630000114440920 Vector: pFL64+ (phagemid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 4.763000011444092 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac replicon: pMB1, f1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--3.7, 1.1; ClaI--4.2, 0.5. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR - f1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589 Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827 Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730 Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361 |
| Shipped： | freeze-dried |