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| 产品名称： | pJIR750 JIR2438 |
|---|---|
| 商品货号： | TS169523 |
| Designations： | pJIR750 JIR2438 |
| Depositors： | JI Rood |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5679998397827150 Vector: pJIR750 (plasmid) Promoters: Promoter lac Construction: pJIR418 Marker(s):cmlR Construct size (kb): 6.567999839782715 Features: insert detection: lacZ marker(s): catP other: rep promoter: lac replicon: pIP404 replicon: pMB1 MCS: EcoRI...HindIII |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--4.4, 2.2; ClaI--6.6; EcoRV--6.6. The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI--0.20, 2.50; ClaI--4.99, 5.03; EcoRV--4.72; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 6.39; SmaI--4.98. The chloramphenicol resistance gene (catP), expressed in both C. perfringens and E. coli, was derived from C. perfringens and is inactivated by insertions at the EcoRV site. The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI. Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting expression of antibiotic resistance in both hosts. |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Bannnam TL, Rood JI. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29: 233-235, 1993. PubMed: 8356117 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of E. coli containing the plasmid. |