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| 产品名称： | p184 |
|---|---|
| 商品货号： | TS169590 |
| Designations： | p184 |
| GenBank Number： | S53291 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.86899995803833 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-7Zf Intact vector size: 3.000 Type of vector: phagemid Vector end: EcoRI Vector end: XbaI Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI HindIII BamHI SacI BstXI NsiI Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI HindIII BamHI SacI BstXI NsiI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ADRB3 Gene name: adrenergic, beta-3-, receptor Contains complete coding sequence?: U Localization: 8 p12-p11.1 Tissue: SK-N-MC cells Type of DNA: cDNA Insert 5 end: EcoRI Insert 3 end: XbaI Insert size (kb): 0.869 Cross references: DNA Seq. Acc.: S53291 Insert lengths(kb): 0.8690000176429749 Tissue: SK-N-MC cells Gene product: adrenergic, beta-3-, receptor ADRB3 |
| Insert Size (kb)： | 0.869 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.5, 0.4; BglI--1.54, 1.32, 0.9, 0.04; EcoRI--3.87; EcoRI/XbaI--3.0, 0.87; NcoI--3.44, 0.43. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.36; BglI--0.07, 0.11; NcoI--0.29, 0.72; SacI--0.55, 0.78. Corresponds to nt 1137-2006 of GenBank accession X70811, spanning a region that contains an intron in the genomic sequence. Constructed after amplification from cDNA with these primer/adapters: 5-TGCGAATTCTGCCTTCAACCCGCTC-3; 5-ACTATAGGGTCTAGAGGATCCG-3. The product was digested with EcoRI + XbaI. Transcription from the SP6 promoter gives the sense strand. |
| References： | Granneman JG, et al. Rodent and human beta 3-adrenergic receptor genes contain an intron within the protein-coding block. Mol. Pharmacol. 42: 964-970, 1992. PubMed: 1336117 James G Granneman, personal communication |