微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pGEM-hIRF |
|---|---|
| 商品货号： | TS169702 |
| Designations： | pGEM-hIRF |
| GenBank Number： | Z11559 |
| Species： | Homo sapiens, human |
| Depositors： | LC Kuhn |
| Vector： | Construct size (kb): 6.498000144958496 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-7Zf(-) Intact vector size: 3.000 Type of vector: phagemid Vector end: EcoRI Vector end: KpnI Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI HindIII BamHI SacI BstXI NsiI Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI HindIII BamHI SacI BstXI NsiI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IREB1 Other gene symbols: IRE1; FRP*; IRF* Gene name: iron-responsive element binding protein 1 Contains complete coding sequence?: Y Chromosome: 9; Localization: 9 Strain: fibroblast cell line EK4 Tissue: placenta Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 3.498 Cross references: DNA Seq. Acc.: Z11559 Insert lengths(kb): 3.497999906539917 Tissue: placenta Gene product: aconitase(iron-responsive element binding protein 1) FRP* |
| Insert Size (kb)： | 3.498 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI/ClaI--3.55, 3.0; EcoRI--3.1, 1.35, 1.3, 0.7; PstI--6.55; BamHI--3.4, 3.15; HindIII--6.2, 0.5. The insert contains the following restriction sites (bp from the 5 end): BamHI--143; BglI--712, 2262, 2493, 2854; EcoRI--1368, 2015, 2220; HindIII--3040; KpnI--2384; PstI--364; PvuII--2154, 3104. A probe derived from sequence between nt 44 and 344 detects a single genomic EcoRI fragment of approximately 4.3 kb in human placental DNA. Contains 107 bp of 5 untranslated region, the complete coding sequence and 720 bp of 3 untranslated region. Detects a single mRNA of approximately 4.0 kb in the fibroblast cell line EK4. Constructed from clones isolated from placenta and fibroblast libraries. |
| References： | Hirling H, et al. Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/translation. Nucleic Acids Res. 20: 33-39, 1992. PubMed: 1738601 |