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| 产品名称： | Der p1 - clone 3e |
|---|---|
| 商品货号： | TS169720 |
| Designations： | Der p1 - clone 3e |
| GenBank Number： | S66377 |
| Species： | Dermatophagoides pteronyssinus, house dust mite (HDM) |
| Depositors： | NA Kalsheker |
| Applications： | contains sequence cysteine protease |
| Vector： | Construct size (kb): 3.349999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: BamHI Vector end: XbaI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: house dust mite (HDM) Gene symbol: Der p1 Gene name: cysteine protease Contains complete coding sequence?: U Type of DNA: cDNA Insert end: Modification: BamHI linker Insert end: Modification: XbaI linker Insert size (kb): 0.7 Cross references: DNA Seq. Acc.: S66377 Nucleotides 21-686 of the insert correspond to nucleotides ~344-1009 of S66377. Insert lengths(kb): 0.699999988079071 Gene product: cysteine protease Der p1 Target Gene: cysteine protease |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--2.7, 0.7; HindIII--3.0, 0.35; PvuII--2.3, 0.75, 0.3. Insert contains the following restriction sites (approximate kb from the 5 end): ClaI--0.06, 0.30, 0.64; HindIII--0.41; NdeI--0.67; PstI--0.03; PvuII--0.05. Insert contains the complete coding sequence for the mature peptide plus the following 20 bp sequence at the 5 end: 5 - CTAATGTGATTTCCAAGCGC - 3. |
| References： | Scobie G, et al. Expression cloning of a dust mite cysteine proteinase, Der p1, a major allergen associated with asthma and hypersensitivity reactions. Biochem. Soc. Trans. 22: 448S, 1994. PubMed: 7698465 Chua KY, et al. Sequence polymorphisms of cDNA clones encoding the mite allergen Der p I. Int. Arch. Allergy Immunol. 101: 364-368, 1993. PubMed: 8353459 Noor A Kalsheker, personal communication |