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| 产品名称： | mKV1.3-EC |
|---|---|
| 商品货号： | TS169728 |
| Designations： | mKV1.3-EC |
| GenBank Number： | M30441 |
| Species： | Mus musculus, mouse |
| Depositors： | G Chandy |
| Applications： | construct to generate mRNA that can be expressed in Xenopus oocytes potassium voltage-gated channel, shaker-related subfamily, member 3 potassium voltage-gated channel, shaker related subfamily, type n |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: genomic Insert lengths(kb): 1.76800000667572 Gene product: potassium voltage-gated channel, shaker-related subfamily, member 3(potassium voltage-gated channel, shaker related subfamily, type n) Kcna3 Target Gene: potassium voltage-gated channel, shaker-related subfamily, member 3 |
| Insert Size (kb)： | 1.768 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII/BstEII--3.2, 1.8; BstEII--5.0; BglII--5.0; BamHI--5.0; EcoRI--5.0. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--0.00; BanI--0.02; NcoI--0.44; PstI--1.02; SacII--0.15. For in vitro transcription, linearize with EcoRI and use SP6 RNA polymerase. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Grissmer S, et al. Expression and chromosomal localization of a lymphocyte K+ channel gene. Proc. Natl. Acad. Sci. USA 87: 9411-9415, 1990. PubMed: 2251283 Krieg PA, Melton DA. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Res. 12: 7057-7070, 1984. PubMed: 6207484 George Chandy, personal communication |