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| 产品名称： | pAL7 |
|---|---|
| 商品货号： | TS169807 |
| Designations： | pAL7 |
| Depositors： | H Okayama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.5000000000000000 Vector: pAL7 (plasmid) Construction: pFL20, pDB262 Marker(s):LEU2,ampR Construct size (kb): 6.5 Features: marker(s): ampR, LEU2 replicon: pMB1, ars |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.3, 1.2, 0.9; HindIII--6.6; PstI--6.6. Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA. Shares pBR322 sequences from the PvuII site to the EcoRI site with pcD. Contains the ars and stb sequences from Schizosaccharomyces pombe and the LEU2 gene from Saccharomyces cerevisiae. The PstI site interrupts the beta-lactamase gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Okazaki K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18: 6485-6489, 1990. PubMed: 2251111 |
| Shipped： | freeze-dried |