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| 产品名称： | pEA500 |
|---|---|
| 商品货号： | TS169963 |
| Designations： | pEA500 |
| Depositors： | SL Forsburg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.3000001907348630 Vector: pEA500 (plasmid) Promoters: Promoter T3 Construction: pEA2, pSP2 Marker(s):ampR,his7+ Construct size (kb): 8.300000190734863 Features: insert detection: lacZ marker(s): ampR marker(s): his7+ promoter: T7, T3 replicon: ars1 replicon: pMB1 MCS: KpnI...SacII |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.3; XhoI--8.3; HindIII--5.3, 1.6, 1.4. Plasmid pEA500 is a cloning vector that carries his7+ and ars1, yet retains the ability to use the blue/white color screen to identify recombinants. The vector was constructed by inserting the PstI-SmaI fragment of pEA2 into plasmid pSP2 digested with NsiI and StuI (both recognize unique sites within the URA3 gene). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169 |
| Related Products： | component of:ATCC 87686 |
| Shipped： | freeze-dried |