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| 产品名称： | Rubella virus |
|---|---|
| 商品货号： | TS170070 |
| Classification： | Togaviridae, Rubivirus |
| Agent： | Rubella virus |
| Strain： | M33 |
| Common Name： | Rubella virus |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Throat washings from Army recruit, New Jersey, 1961 |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Rubella virus is 40-80 nm in diameter and ether sensitive. Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus. |
| Effect on Host： | No CPE produced on production host. Recognized primarily by interference for other viruses (GMK), or production of CF and HA antigens. |
| Recommended Host： | BS-C-1 (ATCC® CCL-26™) |
| Growth Conditions： | Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 11-15 days |
| Name of Depositor： | PD Parkman, Walter Reed Army Institute of Research |
| Source： | Throat washings from Army recruit, New Jersey, 1961 |
| Year of Origin： | 1961 |
| References： | Parkman PD, et al. Recovery of rubella virus from army recruits. Proc. Soc. Exp. Biol. Med. 111: 225-230, 1962. PubMed: 13941530 |