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| 产品名称： | pSE3.2-L |
|---|---|
| 商品货号： | TS170101 |
| Designations： | pSE3.2-L |
| Species： | Homo sapiens, human |
| Depositors： | HF Willard |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: DNA Segment, single copy DXS16 Alleles: B1, B1, B2, B2, A1, A2 |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Digest with HindIII will release two insert fragments of 1.5 and 1.7 kb. The vector is 3.0 kb. Restriction digests of the clone give the following sizes (kb): HindIII--2.9, 1.8, 1.7; PvuI--uncut; BamHI--3.7, 2.4; SalI--3.3, 2.9; EcoRI/HindIII--2.9, 1.8, 1.7; consistent with deposited DNA. This probe is 2 cM (LOD score = 14.96) from pD2 (DXS43), 3 cM (LOD score = 8.56) from 782 (DXS85) and 15 cM (LOD score = 6.05) from dic56 (DXS143). Codominant, X-linked segregation demonstrated in several large, 3-generation families. Low background with final wash in 0.1X SSC at 65C. Obtained from a recombinant lambda phage identified by a chromosome walk from pXUT23 (ATCC 57154/5). This is 22 kb from pXUT23. Enzyme(s) not detecting polymorphism: BamHI, BglII, HindiII, TaqI in a screen of 4 female Caucasians. |
| References： | Brown CJ, Willard HF. MspI RFLP detected with chromosome-walk clone pXUT23-SE3.2L from DXS16 in Xp22.1-22.3. Nucleic Acids Res. 15: 9614, 1987. PubMed: 2446265 Huntington F Willard, personal communication |