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| 产品名称： | cosmid322 |
|---|---|
| 商品货号： | TS170116 |
| Designations： | cosmid322 |
| GenBank Number： | L01971 L01972 L01973 L01974 L01975 L01976 L01977 L01978 L01979 L01980 L01981 L01982 L01983 |
| Species： | Homo sapiens, human |
| Depositors： | JF Gusella |
| Vector： | Construct size (kb): 52.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pWE15 Intact vector size: 8.200 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI Polylinker sites: EcoRI NotI BamHI NotI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, kanR, G418R promoter: T3, T7 replicon: pMB1 Cross references: DNA Seq. Acc.: X65279 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SCN4A Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide Contains complete coding sequence?: N Chromosome: 17; Localization: 17 q23.1-q25.3 Tissue: lymphoblast Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 44.0 Cross references: DNA Seq. Acc.: L01971 DNA Seq. Acc.: L01972 DNA Seq. Acc.: L01973 DNA Seq. Acc.: L01974 DNA Seq. Acc.: L01975 DNA Seq. Acc.: L01976 DNA Seq. Acc.: L01977 DNA Seq. Acc.: L01978 DNA Seq. Acc.: L01979 DNA Seq. Acc.: L01980 DNA Seq. Acc.: L01981 DNA Seq. Acc.: L01982 DNA Seq. Acc.: L01983 Insert lengths(kb): 44.0 Tissue: lymphoblast Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide SCN4A |
| Insert Size (kb)： | 44.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--13.0, 10.0, 6.6, 4.8, 4.0, 3.4 (doublet), 0.6; BglII--13.5, 7.0 (doublet), 5.1, 4.2, 3.1, 1.7, 1.6, 1.45; EcoRI--17.0, 9.2, 9.0, 5.9, 2.9, 1.1; HindIII--17.5, 9.4, 5.8, 3.5, 3.3, 2.95, 0.45; SstI--11.5 (doublet), 4.9, 3.3, 2.95, 2.4 (doublet), 1.25, 1.1, 0.80, 0.62, 0.45, 0.30. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Includes exons 12 - 24, with exon 24 (and thus the 3 untranslated region) being complete. This corresponds to domains II, III and IV of the protein product. Additional GenBank/EMBL accession numbers: L01971-L01982. |
| References： | McClatchey AI, et al. The genomic structure of the human skeletal muscle sodium channel gene. Hum. Mol. Genet. 1: 521-527, 1992. PubMed: 1339144 |