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| 产品名称： | pHIL6R.32 |
|---|---|
| 商品货号： | TS170128 |
| Designations： | pHIL6R.32 |
| GenBank Number： | M20566 |
| Species： | Homo sapiens, human |
| Depositors： | GH Fey |
| Applications： | The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 440 to 1649 of the published sequence. |
| Vector： | Construct size (kb): 4.169000148773193 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.205000042915344 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: interleukin 6 receptor IL6R |
| Insert Size (kb)： | 1.205 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert includes the following restriction sites (in nucleotides from the 5 end of the published sequence): SmaI--580, PstI--638, SacI--1201. Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.9, 1.2; HindIII--3.9, 0.9; BamHI--4.1; PstI--3.15, 1.0; SalI--4.1; EcoRI/XbaI--2.9, 1.3; BamHI/XhoI--2.9, 1.3. The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 440 to 1649 of the published sequence. It encodes most of the protein coding region (437 - 1841). The insert is oriented such that linearizing with BamHI and using the T3 promoter gives the antisense strand, and linearizing with EcoRI and using the T7 promoter gives the sense strand. |
| References： | Baumann M, et al. Molecular cloning, characterization and functional expression of the rat liver interleukin 6 receptor. J. Biol. Chem. 265: 19853-19862, 1990. PubMed: 2174054 Yamasaki K, et al. Cloning and expression of the human interleukin-6 (BSF-2/IFN-beta2) receptor. Science 241: 825-828, 1988. PubMed: 3136546 Georg H Fey, personal communication |