Construct size (kb): 5.300000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HNRPK
Gene name: heterogenous nuclear ribonucleoprotein K
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.3
Cross references: DNA Seq. Acc.: T07375; EST: EST05264; Primer: M13-21; THC
id: THC122448
DNA Seq. Acc.: X72727; EST: EST05264
Nucleotides 1-311, 287-360 of the insert correspond to
nucleotides 2277-1967, 1992-1929 of X72727 with 95% identity.

Insert lengths(kb): 2.299999952316284

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: heterogeneous nuclear ribonucleoprotein complex K HNRPK

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.3.

Based on the alignment with GenBank X72727 (2830 nt, CDS 210-1604) and the insert size, this clone appears to contain most if not all of the coding sequence.