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| 产品名称： | 4.25 plasmid in E. coli |
|---|---|
| 商品货号： | TS170271 |
| Designations： | 4.25 plasmid in E. coli |
| Species： | Tobacco mosaic virus |
| Depositors： | WO Dawson |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.32 Gene product: end, 3 |
| Insert Size (kb)： | 1.320 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains the following sites separated by (bp)(approx): HindIII- 195- PvuII- 780- AccI- 350- PstI. Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PstI--4.0; HindIII/PstI--2.7, 1.35. The insert spans nucleotides 5080-6395 of TMV. |
| Classification： | Tobamovirus |
| References： | Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986. |