| 产品名称： |
Oxytricha trifallax |
| 商品货号： |
TS170355 |
| Strain Designations： |
SLC 89 |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： |
Unknown |
| Product Format： |
frozen |
| Storage Conditions： |
Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section |
| Type Strain： |
no |
| Medium： |
ATCC® Medium 802: Sonneborns Paramecium medium
|
| Growth Conditions： |
Temperature: 20°C to 25°C
Culture system: Xenic, with Enterobacter aerogenes ATCC 13048 and unidentified microalgae as food sources |
| Cryopreservation： |
Reagents
Cryoprotective Solution
DMSO, 2.0 mLxa0
Fresh growth medium w/o bacteria, 8.0 mL
Harvest and Preservation
- Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.xa0
- Harvest cells from a culture that is at or near peak density by centrifugation at 125 x g for 5 min.xa0
- Adjust the concentration of cells at least 1 x 105/mL in fresh medium.xa0
- Mix the cell preparation and the cryoprotective solution in equal portions.xa0
- Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
- Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.xa0
- Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.xa0
- To establish a culture from the frozen state add 1.0 mL bacterized ATCC medium 802 to the frozen ampule and place it in a 35°C water bath. xa0Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0
- Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 mL of bacterized ATCC medium 802.xa0
- Incubate at 25°C with the cap on loosely.xa0
- Once the culture is established, transfer 0.5 mL to 5.0 mL of bacterized ATCC medium 802.xa0
- Follow the protocol for maintenance of culture.
|
| Name of Depositor： |
GA Herrick |
| Geographical Isolation： |
unknown |
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