微信小陈
微信小章
产品简介
购买须知
| 产品名称： | p253 |
|---|---|
| 商品货号： | TS170421 |
| Designations： | p253 |
| GenBank Number： | J00134 |
| Species： | Homo sapiens, human |
| Depositors： | ST Lord |
| Applications： | produces protein fibrinogen, gamma polypeptide |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Tissue: liver Gene product: fibrinogen, gamma polypeptide FGG Alleles: A1, A2 Target Gene: fibrinogen, gamma polypeptide |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--6.2; EcoRI--6.2; HindIII/EcoRI--5.0, 1.2; HpaI--6.2; BamHI--6.2. The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region. Gamma genomic DNA sequence is available from GenBank and includes the following numbers: J00134, J00135, M10014, X00086. Expresses intact fibrinogen gamma chain which cross-reacts with antisera and retains the ability to clump cells of Staphylococcus aureus Newman D(2)C. The polymorphism is at a KpnI site; the SacI digest is used to decrease the fragment sizes to detectable lengths. The ability of this probe to detect the KpnI polymorphism was verified by J. Murray, University of Iowa. |
| References： | Bolyard MG, Lord ST. High-level expression of a functional human fibrinogen gamma chain in Escherichia coli. Gene 66: 183-192, 1988. PubMed: 3049244 Susan T Lord, personal communication |