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| 产品名称： | HIBBR61 |
|---|---|
| 商品货号： | TS170446 |
| Designations： | HIBBR61 |
| GenBank Number： | Z22555 T09272 T09273 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD36L1 Other gene symbols: CLA-1 Gene name: CD36 antigen (collagen type I receptor, thrombospondin receptor) - like 1 Contains complete coding sequence?: N Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: NotI Insert size (kb): 2.0 Cross references: DNA Seq. Acc.: T09272; EST: EST07165; Primer: M13-21; THC id: THC124116 DNA Seq. Acc.: T09273; EST: EST07166; Primer: M13 Reverse; THC id: THC124116 DNA Seq. Acc.: Z22555 Nucleotides 1-365 of the insert correspond to nucleotides 570-935 of Z22555 with 99% identity. DNA Seq. Acc.: Z22555; EST: EST07165 Nucleotides 303-215, 214-2 of the insert correspond to nucleotides 2252-2340, 2340-2552 of Z22555 with 98% identity. Insert lengths(kb): 2.0 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: scavenger receptor class B type I( CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1, scavenger receptor class B type 1) CLA-1 |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.0, 2.0; EcoRI--3.5, 1.5. Verified to contain EST07165 by single-pass sequencing. Based on the alignment with GenBank Z22555 (2566 nt, CDS 70-1599), this clone appears to contain part of the coding sequence and the 3 untranslated region. The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII. |
| References： | Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585 Mark D Adams, personal communication |