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| 产品名称： | pVT65 |
|---|---|
| 商品货号： | TS170500 |
| Designations： | pVT65 |
| Depositors： | VN Trieu |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM109 |
| Vector Information： | Size (kb): 6.5000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 6.500 Type of vector: plasmid Cloning sites: PstI HincII PvuI NcoI ScaI SspI BalI Polylinker sites: Construction: pDM2, pBGS8 Host range: Haemophilus influenzae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, cmLR replicon: pMB1, pRSF0885 Vector: pVT65 (plasmid) Construction: pDM2, pBGS8 Marker(s):ampR,cmlR Construct size (kb): 6.5 Features: marker(s): ampR, cmlR replicon: pMB1, pRSF0885 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--6.6; PvuI--6.6; SspI--3.7, 2.9; PvuII--6.2, 0.46; BamHI--4.0, 2.4. Cloning into the HincII, PstI, PvuI, ScaI, or SspI sites inactivates ampR. Cloning into the BalI or NcoI sites inactivates cmlR. Precautions have not been taken to maintain the F in the JM109 host. The F is not required to use this plasmid. Shuttle vector. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Trieu VN, McCarthy D. Improved plasmid shuttle vectors for Haemophilus influenzae and Escherichia coli. Gene 86: 99-102, 1990. PubMed: 2179060 Vuong N Trieu, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |