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| 产品名称： | pAV2 |
|---|---|
| 商品货号： | TS170510 |
| Designations： | pAV2 |
| Depositors： | CA Laughlin |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.7
Vector: pAV2 (plasmid)
Promoters: Promoter P19
Construction: pA11P.Xba, AAV genome
Marker(s):ampR
Construct size (kb): 8.7
Features: marker(s): ampR promoter: viral P5, P19, P40 replicon: pMB1, AAV terminator: AAV polyadenylation |
| Applications： | contains sequence human adeno-associated virus type 2 expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--4.7, 3.7; EcoRI--6.6, 1.8. pAV1 (ATCC 37215) and pAV2 (TS170510) both contain the complete AAV2 genome, but in opposite orientations. The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted). Contains the complete viral genome for use in site-specific mutagenesis studies. Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Laughlin CA, et al. Cloning of infectious adeno-associated virus genomes in bacterial plasmids. Gene 23: 65-73, 1983. PubMed: 6352411 Wondisford FE, et al. Cloning of the human thyrotropin beta-subunit gene and transient expression of biologically active human thyrotropin after gene transfection. Mol. Endocrinol. 2: 32-39, 1988. PubMed: 3398841 |
| Shipped： | frozen |
| Shipping Information： | Distributed: frozen |